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. 2023 Aug 19;12(16):2105. doi: 10.3390/cells12162105

Figure 1.

Figure 1

Expansion parameters and basic characterization of MSCs grown in media 1 to 13. MSCs, primarily isolated in medium αMEM+8%PL, were expanded in media 1 to 13 (for composition see Table 1; colors as indicated in scheme) for P3. The expansion parameters harvesting density (A), doubling time (B), and number of population doublings (C) were determined in addition to the viability of cells (D). The expression of identity markers (CD73, CD90, and CD105) (E) and purity markers (CD14, CD34, CD45, and MHC II) (F) was analyzed by flow cytometry. Data are presented as mean ± SD and N ≥ 3 independent experiments were performed. Statistically significant differences are depicted as follows: *: p < 0.05; **: p < 0.01; ***: p < 0.001.