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. 2023 Aug 19;12(16):2105. doi: 10.3390/cells12162105

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expansion parameters and basic characterization of MSCs grown in media 1, 4, 7, 10, and 13. MSCs were isolated and subsequently expanded in media 1 (100% αMEM+8%PL; black), 4 (95% αMEM+8%PL + 5% StemMACS^TM; blue), 7 (50% αMEM+8%PL + 50% StemMACS^TM; green), 10 (5% αMEM+8%PL + 95% StemMACS^TM; red) and 13 (100% StemMACS^TM; violet) for P1. The expansion parameters harvesting density (A), doubling time (B), and number of population doublings (C) were determined in addition to the viability of cells (D). The expression of identity markers (CD73, CD90, and CD105) (E) and purity markers (CD14, CD34, CD45, and MHC II) (F) was analyzed by flow cytometry. Data are presented as mean ± SD and N ≥ 4 independent experiments were performed. Statistically significant differences are depicted as follows: *: p < 0.05; **: p < 0.01; ***: p < 0.001.