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. 2023 Aug 19;12(16):2105. doi: 10.3390/cells12162105

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Surface antigen expression by MSCs grown in media 1, 4, 7, 10, and 13. MSCs grown in media 1 (100% αMEM+8%PL; black), 4 (95% αMEM+8%PL + 5% StemMACS^TM; blue), 7 (50% αMEM+8%PL + 50% StemMACS^TM; green), 10 (5% αMEM+8%PL + 95% StemMACS^TM; red), and 13 (100% StemMACS^TM; violet) were analyzed for the expression of different surface antigens. These included metabolism-related markers GLUT1, GLUT3, GLUT4, FGFR1, FGFR2, FGFR3, PDGFRA, PDGFRB, INSR, IGF1R, IGF2R, STRA6, NGFR, and EGFR (A), cell adhesion-related markers CD29, CD49a, CD49c, CD49d, CD49e, CD49f, CD51, CD61, CD31, CD44, and CD146 (B), tetraspanins CD9, CD63, and CD81 (C), as well as the additional markers CD3, CD36, CD362, CD10, CD13, MHC I, and MSCA1 (D). Data are presented as mean ± SD and N ≥ 3 independent experiments were performed. Statistically significant differences are depicted as follows: *: p < 0.05; **: p < 0.01; ***: p < 0.001.