Cell viability of human small intestinal epithelial cells treated with transient receptor potential channel ligands. Graphs show the cell viability after 24, 48, 72, and 96 h of exposure to (A) capsaicin, (B) curcumin, or (C) eugenol. Cell viability was quantified by the MTT assay with the absorbance at 595 nm. Data were analyzed with one-way ANOVA followed by a Dunnett’s post hoc test compared to the control group. Bars represent the mean ± SD. Significance is indicated using the following scale: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. Cap, capsaicin; Cur, curcumin; Eug, eugenol.