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. 2023 Aug 14;12(16):2062. doi: 10.3390/cells12162062

Figure 6.

Figure 6

Differential effects of repetitive transcranial magnetic stimulation and fluoxetine on the immunoreactivity of the histone H2B (K16) acetylation and histone H3 trimethylation. (A) Representative micrographs at 10× (scale bar = 400 µm) and 40× (scale bar = 100 µm) of the histone H2B acetylation in lysine 16 (H2BK16ac) immunolabeling in layer II/III of the frontal cortex (FC) (A1–8) of the experimental groups Ctrl (A1, 2), sham (A3, 4), rTMS (A5, 6), and FLX (10 mg/Kg) (A7, 8). (B) FLX increased the H2BK16ac immunoreactivity compared with the Ctrl, sham, and rTMS groups in the FC (which was determined using the one-way ANOVA followed by the Bonferroni post hoc test). (C) Representative micrographs at 10× (scale bar = 400 µm) and 40× (scale bar = 100 µm) of the histone H2B acetylation in lysine 16 (H2BK16ac) immunolabeling in the dentate gyrus (DG) of the hippocampus (C1–8) of the experimental groups Ctrl (C1, 2), sham (C3, 4), rTMS (C5, 6), and FLX (10 mg/Kg) (C7, 8). (D) Stress and the therapeutic interventions did not modify the H2BK16ac immunoreactivity in the DG (one-way ANOVA). (E) Representative micrographs at 10× (scale bar = 400 µm) and 40× (scale bar = 100 µm) of the histone H3 trimethylation in lysine 9 (H3K9me3) immunolabeling in the layer II/III of the FC (E1–8) of the experimental groups Ctrl (E1, 2), sham (E3, 4), rTMS (E5, 6), and FLX (10 mg/Kg) (E7, 8). (F) Stress does not modify the H3K9me3 immunoreactivity but the rTMS increased it in the FC (which was determined using the one-way ANOVA followed by the Bonferroni post hoc test). (G) Representative micrographs at 10× (scale bar = 400 µm) and 40× (scale bar = 100 µm) of the histone H3 trimethylation in lysine 9 (H3K9me3) immunolabeling in the ML of the hippocampus (G1–8) of the experimental groups Ctrl (G1, 2), sham (G3, 4), rTMS (G5, 6), and FLX (10 mg/Kg) (G7, 8). (H) Stress and the therapeutic interventions do not modify the H3K9me3 in the DG (one-way ANOVA). In all panels * represents a significant difference between groups (* p ≤ 0.05, ** p ≤ 0.005, *** p ≤ 0.001). Error bars represent the standard error of the mean. n = 4. A.U—arbitrary units.