Figure 3.

Impact of P. regalis venom on the fusion of myoblasts into myotubes and atrophy of myotubes. (A) Schematic representation of the protocol used for fusion assay. (B) Staining of myotubes for MHC1 on day 7 (affected areas are indicated by arrows). (C) Quantification of the fusion index of the myotubes (n = 11). (D) Schematic representation of the protocol used for the atrophy assay. (E) Staining of myotubes for MHC1 on day 8. (F) Quantification of the myotube area (n = 20). Data represent the mean ± SEM. p values shown were calculated by one-way ANOVA followed by the Bonferroni post-test (* p < 0.05, and *** p < 0.001). The scale bars represent 200 µm, and they are applicable for all images in each panel.