Figure 6.

Impact of P. regalis venom in inducing bleeding and blood clots in the tibialis anterior muscle in mice. (A) Localisation of the P-selectin exposure on the surface of platelets in muscle sections. The graph shows the quantification of the total microthrombi area per 200 µm² view field (n = 24 images and 5 mice for each cohort). (B) Localisation of fibrinogen in muscle sections and the graph shows the quantification of percentage fibrinogen coverage per damaged area with representative images showing areas of 200 µm² (n = 5 mice for each cohort). Data represent the mean ± SEM. p values (* p < 0.05 and *** p < 0.001) were calculated by one-way ANOVA followed by the Bonferroni post-test. The arrows indicate the areas with microthrombi in muscle sections. The scale bars represent 50 µm. ‘C’ represents the contralateral control muscles that were collected on day 10.