FIG. 2.

The two monoclonal antibodies (C179 and F49) and anti-influenza B virus rabbit serum stained the infected cells in a subtype- or type-specific manner. The MDCK cells in 96-well flat bottom plates were infected with A/Osaka/219/91 (A, B, and C), A/Osaka/174/85 (D, E, and F), and B/Osaka/753/93 (G, H, and I) and were stained with C179 (A, D and G), F49 (B, E and H), and anti-influenza B virus rabbit serum (C, F, and I). The cells infected with A/Osaka/219/91 were stained with C179 but not with F49 or anti-influenza B virus rabbit serum, which confirmed that the strain was H1N1. Similarly, A/Osaka/174/85 and B/Osaka/753/93 were identified to be H3N2 and the B type, respectively.