Figure 2.

Effect of electrotransfer buffer formula and different electrotransfer times on different molecular weight proteins. (A) Replacing methanol with ethanol in the electrotransfer buffer achieved the same efficiency. The experimental procedure was the same as the standard experimental procedure except for the electrotransfer buffer. (B) Signal intensities of different electrotransfer buffers were statistically analyzed and compared (n = 6). (C) The effect of different electrotransfer times on the signal intensities of proteins with different molecular weights. The experimental procedure was the same as the standard experimental procedure except for the electrotransfer time. (D) Signal intensities of different electrotransfer times were statistically analyzed and compared (n = 6).