(a) The schematic illustration for branched DNA (Y1 and Y2-DNA) synthesis. Y-DNA was prepared by annealing three ssDNA. (b) The hybridization between probe and amplifier triggered by T-DNA. (c) The assay platform based on branched DNA and AuNPs-branched DNA. (d) Cyclic voltammetry responses, (e) DPV response, and (f) Nyquist plots. The solid lines a, b, c, d, and e in (d–f) represented the signal from bare gold electrode, Y1-DNA-modified gold electrode, Y1-DNA-modified gold electrode, and the detection of T-DNA, amplifier on the Y2-DNA modified gold electrode in 0.1 M KCl solution containing 5 mM [Fe(CN)6]3−/4−].