Figure 6.

The role of TRIM14 overexpression in the growth and metastasis of HCC cells in vivo. HCC cells (HCC-LM3 and Huh-7) transfected with negative vector or TRIM14 overexpression plasmid were subcutaneously inoculated into nude mice. On Day 30 after treatment, the nude animals (n = 5 in each group) were killed via cervical dislocation. (A–C) The tumor volume (A), images (B), and weight (C). (D) The lung metastasis amount was determined through caudal vein injection, and HE staining was used to evaluate lung metastasis of tumor cells. (E–H) Immunohistochemistry confirmed TRIM14 (E), Vimentin (F), E-cadherin (G), and N-cadherin (H) expression. Scale bar = 100 μm. (I) Western blot analysis of the profiles of EMT-associated proteins (E-cadherin, N-cadherin, Snail, and Vimentin) in tumor tissues. ** p < 0.01 and *** p < 0.001 (vs. the vector/Si-NC group). N = 5.