Table 1.

Primers and probes used to assess the transcription levels of pol genes of HERV-K, -W, and –H; env genes of Syncytin 1, Syncytin 2, and HERV-W; TRIM28 and SETDB1; and GAPDH.

Name	Primer/ Probe	Sequence
HERV-H pol	Forward	5′-TGGACTGTGCTGCCGCAA-3′
	Reverse	5′-GAAGSTCATCAATATATTGAATAAGGTGAGA-3′
	Probe	6FAM-5′-TTCAGGGACAGCCCTCGTTACTTCAGCCAAGCTC-3′-TAMRA
HERV-K pol	Forward	5′-CCACTGTAGAGCCTCCTAAACCC-3′
	Reverse	5′-TTGGTAGCGGCCACTGATTT-3′
	Probe	6FAM-5′-CCCACACCGGTTTTTCTGTTTTCCAAGTTAA-3′-TAMRA
HERV-W pol	Forward	5′-ACMTGGAYKRTYTTRCCCCAA-3′
	Reverse	5′-GTAAATCATCCACMTAYYGAAGGAYMA-3′
	Probe	6FAM-5′-TYAGGGATAGCCCYCATCTRTTTGGYCAGGCA-3′-TAMRA
Syncytin 1 env	Forward	5′-ACTTTGTCTCTTCCAGAATCG-3′
	Reverse	5′-GCGGTAGATCTTAGTCTTGG-3′
	Probe	6FAM-5′-TGCATCTTGGGCTCCAT-3′-TAMRA
Syncytin 2 env	Forward	5′-GCCTGCAAATAGTCTTCTTT-3′
	Reverse	5′-ATAGGGGCTATTCCCATTAG-3′
	Probe	6FAM- 5′-TGATATCCGCCAGAAACCTCCC-3′-TAMRA
HERV-W env	Forward	5′-CTTCCAGAATTGAAGCTGTAAAGC-3′
	Reverse	5′-GGGTTGTGCAGTTGAGATTTCC-3′
	Probe	6FAM-5′-TTCTTCAAATGGAGCCCCAGATGCAG-3′-TAMRA
TRIM28	Forward	5′-GCCTCTGTGTGAGACCTGTGTAGA-3′
	Reverse	5′-CCAGTAGAGCGCACAGTATGGT-3′
	Probe	6FAM-5′-CGCACCAGCGGGTGAAGTACACC-3′-TAMRA
SETDB1	Forward	5′-GCCGTGACTTCATAGAGGAGTATGT-3′
	Reverse	5′-GCTGGCCACTCTTGAGCAGTA-3′
	Probe	6FAM-5′-TGCCTACCCCAACCGCCCCAT-3′-TAMRA
GAPDH	Forward	5′-CGAGATCCCTCCAAAATCAA-3′
	Reverse	5′-TTCACACCCATGACGAACAT-3′
	Probe	6FAM-5′-TCCAACGCAAAGCAATACATGAAC-3′-TAMRA