Figure 6.

SIRT1/FoxO1 activation by melatonin promotes mitophagy in bovine ovarian granulosa cells. (A,B) The relative protein level of FoxO1 was detected by Western blot (n = 3). ** p < 0.01 vs. Control group. ## p < 0.01 vs. H₂O₂ group.(C,D) The relative protein level of FoxO1 was detected after treatment with EX527 (n = 3). (E,F) The relative protein level of FoxO1 was detected after overexpression of FoxO1 (n = 3). (G,H) The relative protein levels of PINK1, PARKIN, BECLIN1, SQSMT1, LC3I, and LC3II were detected by Western blot (n = 3). (I,J) The percentage of mitophagy-positive cells was detected by double staining of lyso dye (green) and mtphage dye (red). The values are shown as mean ± SD. * p < 0.05, ** p < 0.01, or *** p < 0.001 vs. MT+H₂O₂ group.