Figure 1.

NASH was induced in the STAM^TM model. (A) The gross phenotype of the liver. (B) Liver weight (upper) and liver weight/body weight (lower). (C) Masson’s trichrome staining of liver tissue. (D) Scores indicating NAFLD activity. (E) Western blot data showing the expression levels of the inflammatory factors IL-6 and F4/80. (F) Assays showing that insulin resistance was induced in the STAM^TM model. GTT (upper) and ITT (lower). (G) Assays showing that hemolysis occurred in the serum of NASH mice. Photograph of serum showing hemolysis, quantification of hemoglobin, heme assay, and hemopexin ELISA. ** p < 0.01, Mann–Whitney U test.