Table 1.

Oligonucleotides used for RT-PCR analysis and calculated size of the products.

Genes Accession No.	Primer No.	Primer Pairs	Nucleotide Positions	Annealing T Size (bp)
Agr2 NM_011783.2	MB2190 MB2191	GTCTGCAATCCTGCTTCTTGT GTCTTTAGCAGCTTGAGAGCTT	70–90 570–549	60 °C 501
Cdx1 NM_009880.4	MB2354 MB2355	GGACGCCCTACGAATGGAT ACCAGATCTTTACCTGCCGC	489–507 704–685	60 °C 216
Cdx2 NM_007673.3	MB2352 MB2353	AGCCAAGTGAAAACCAGGACA GATGCTGTTCGTGGGTAGGA	799–819 1320–1301	60 °C 522
Clca1 NM_017474.2	MB2176 MB2177	CTTATCACCTGGACAACGCA TGGTCCCTGAGATCAACGAT	1589–1608 2436–2417	60 °C 848
Duox2 NM_001362755.1	MB2726 MB2727	GCCTGTCGAGTCTCGTTCAT CCGCAAGAAGGTGATGAGGT	2934–2953 3383–3364	60 °C 450
Fut2 NM_001271993.1	MB2468 MB2469	CTCCCCCGGGATCCTTATCT GTGGTAATTCTGCCACGGG	252–271 699–681	60 °C 448
Lgr5 NM_010195.2	MB2492 MB2493	GTCTCCTACATCGCCTCTGC AGAAGGGTTGCCTACGAACG	538–557 1133–1114	60 °C 596
Lpo NM_080420.3	MB3005 MB3006	GGCTGCCACGGGAGGTCAA TTATAGGGTGGTGTGGGGCA	11–29 906–887	60 °C 896
Mki67 NM_001081117.2	MB2458 MB2459	AGAGCTAACTTGCGCTGACTG TCTTGAGGCTCGCCTTGATG	129–149 618–599	60 °C 490
Muc2 NM_023566.4	MB2178 MB2179	GGCTCTACAGACAAGCAGAC CATGAAGGTATGGTCAGGGC	1329–1348 2141–2122	60 °C 813
Nox1 NM_172203.2	MB2883 MB2884	AAGTTTCTCTCCCGAAGGACC CCCTCAAGAAGGACAGCAGA	74–94 387–368	60 °C 314
Pdia6 NM_027959.4	MB2991 MB2992	TGGTCGGACGAGATCTGACA TGAGACGCTGAGGTTCACTG	806–825 1513–1494	60 °C 708
Qsox1 NM_001024945.1	MB2546 MB2547	TATAGTGAGGCCCACCCACA GTACATCTAGGGCAGTGGCTC	1370–1389 1895–1875	60 °C 526
Sod1 NM_011434.2	MB2837 MB2838	CGGTGAACCAGTTGTGTTGTC GGTCTCCAACATGCCTCTCT	174–194 349–330	60 °C 176
Sod2 NM_013671.3	MB2839 MB2840	CTGGACAAACCTGAGCCCTA GTTGTTCCTTGCAATGGGTCC	510–529 728–708	60 °C 219
Sod3 NM_011435.3	MB2184 MB2185	CTGCTGCTCGCTCACATAA CGCCTGGAGACATCTATGC	119–137 1077–1059	60 °C 959
Spdef NM_013891.4	MB2200 MB2201	AAGATATTGAGACGGCCTGC TGTCTATCTGGGACCTTGGG	790–809 1528–1509	60 °C 739
Zg16 NM_026918.3	MB2995 MB2996	CCTCGGCCTCTGCTAATTCC CCTGGATCACAGATTCCCCG	85–104 339–320	60 °C 255