Figure 2.

Western blot analysis of total ezrin (A) and phosphorylated ezrin (B) protein expression in vemurafenib-sensitive (parental, RKO) and -resistant (RKOr) colon cancer cells cultured in the absence (CTRL) and presence of vemurafenib (3 µM) for indicated time periods (24 h, 48 h, 72 h). The expression levels of ezrin and phospho-ezrin phosphorylated at threonine 567 were measured by densitometry analysis using the Quantity One 1-D Analysis Software version 4.6.6. The level of ezrin was normalised to the total amount of proteins loaded in the lane (total protein normalisation), whereas the level of phospho-ezrin was normalised to ezrin. The data represent the mean and standard deviation obtained from three independent biological experiments. Statistical significance (p < 0.05) is denoted with an asterisk.