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. 2023 Aug 18;24(16):12936. doi: 10.3390/ijms241612936

Figure 2.

Figure 2

Lack of P2X7 exacerbates intestinal fibrosis in murine heterotopic transplant model. Murine intestinal fibrosis was induced with the heterotopic transplant model. (A) Graph shows mRNA expression of P2X7 receptor in intestinal grafts from both WT and P2X7-/- mice at Day 0 and Day 7 after surgery (n = 6). (B) Heat map showing the mRNA expression of profibrotic markers Col1a1, TGF-β, Vimentin, Snail1, Snail2, E-Cadherin, Timp1, and Mmp2. Graphs show the mRNA expression of these profibrotic markers in intestinal grafts from both WT and P2X7-/- mice at Day 0 and Day 7 after surgery (n = 6). (C) Representative images of intestinal grafts of the four experimental groups after Sirius Red Staining. Graph shows the quantification of the thickness of the collagen layer (n = 6). The length of the scale bar represents 100 µm. In all cases, bars in graphs represent mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. intestinal graft from WT mice at Day 0; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. intestinal grafts from WT mice at Day 7.