Skip to main content
. 2023 Aug 18;24(16):12936. doi: 10.3390/ijms241612936

Figure 4.

Figure 4

Absence of P2X7 receptor alters the expression of proinflammatory, anti-inflammatory, and macrophage markers after murine intestinal fibrosis. Murine intestinal fibrosis was induced by two different models: heterotopic transplant model and chronic administration of DSS in both WT and P2X7-/- mice. (A) Heat map showing the mRNA expression of iNOS, Arginase, Tnf-α, Cox-2, IL-1β, IL-6, IL-8, IL-13, and IL-10 after the heterotopic transplant model. Graphs show the mRNA expression of these genes in intestinal grafts from both WT and P2X7-/- mice at Day 0 and Day 7 after surgery (n = 4). (B) Heat map showing the mRNA expression of iNOS, Arginase, Tnf-α, Cox-2, IL-1β, IL-6, IL-8, IL-13, and IL-10 after the chronic administration of DSS. Graphs show the mRNA expression of these genes in both WT and P2X7-/- mice with and without DSS (n = 4). (C) Heat map showing the mRNA expression of F4/80, Cd86, Ccr7, Cd11c, Cd206, Ym1, Fizz1, Cd16, and Cd163 after the heterotopic transplant model. Graphs show the mRNA expression of these genes in intestinal grafts from both WT and P2X7-/- mice at Day 0 and Day 7 after surgery (n = 4). (D) Heat map showing the mRNA expression of F4/80, Cd86, Ccr7, Cd11c, Cd206, Ym1, Fizz1, Cd16, and Cd163 after the chronic administration of DSS. Graphs show the mRNA expression of these genes in both WT and P2X7-/- mice with and without DSS (n = 4). In all cases, bars in graphs represent mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. WT Day 0 or WT H2O, depending on the murine model of intestinal fibrosis performed; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. WT Day 7 or WT–DSS mice, depending on the murine model of intestinal fibrosis performed.