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. 2023 Aug 16;24(16):12834. doi: 10.3390/ijms241612834

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Western Blot to Detect Human ANKHD1. A representative Western Blot of ANKHD1 when whole cell lysate from either OX161-Cl or SKI001 cells (described in [28]) is used after siRNA knockdown with either control siRNA (siCtrl) or ANKHD1 specific siRNA (siANKHD1). The primary antibodies used were affinity purified ANKHD1 (#HPA008718) antibody at 1:1000 dilution and visualised with anti-rabbit (#P0448) antibody, and beta-actin (#ab8224) antibody used as a loading control at 1:1000 dilution, visualised with anti-mouse (#P0447) antibody and developed using ECL select. The predicted band size of ANKHD1 is 270kDa, with some laddering expected [29,30]. There is a non-specific band at 25 kDa. Western blot created by Dr Fiona Macleod.