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. 2023 Aug 8;24(16):12578. doi: 10.3390/ijms241612578

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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SNORA33 depletion does not affect proliferation or translation capacity. (A) Growth rate of SNORA33-depleted cells measured by crystal violet staining. Data (n = 4) are presented as fold change to Day 0 and were analyzed by two-way ANOVA. (B) The total rate of protein synthesis of SNORA33-depleted cells (n = 3) measured by [³⁵S]methionine/cysteine incorporation. Data are presented as fold changes to CRISPR control. Statistical significance was assessed by unpaired t-test with the assumption of a normal distribution of the data. (C) Polysome profiles of CRISPR control and SNORA33-depleted cells (n = 1).