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. 2023 Aug 11;24(16):12673. doi: 10.3390/ijms241612673

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of the Na-K ATPase blocker ouabain (perfused for about 10–15 min) on the BK current in U251 edge cells. (a) Experimental traces elicited by applying a voltage step depolarizing protocol from a holding potential of −60 mV (protocol shown at the bottom) in the control conditions (left traces) and after the local perfusion of ouabain 500 µM (central traces). The ouabain-sensitive current was obtained by digital subtraction (right traces). (b) Mean I–V relationships (n = 5 cells) recorded in the control conditions (CTR, dots) and the ouabain condition (ouabain, triangles). Insert shows the averaged I–V in the interval range between −40 mV and +40 mV at higher magnification to show the I–V shift after the local perfusion of ouabain.