Figure 2.

Effects on eEV concentration and sizes after eEV storage in PBS and the EV buffer. eEVs were isolated from HEK293T-conditioned medium by differential ultracentrifugation, resuspended in (A) PBS, (B) EV buffer, or (C) Media without isolation and stored at both 4 °C or (D) −80 °C for indicated duration. Effects of storage on EV concentration, size, and volume distribution are shown. Particle number was standardized on yield from 1 mL culture media. The data of PBS Day 0 were used as the data of media day 0. (n = 7). One-way ANOVA was used to evaluate the effect of the time course in the group, and unpaired t-test was used to compare different storage conditions. In all figures, significance against Day 0 is expressed as follows: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, and **** p ≤ 0001, if not otherwise specified.