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. 2023 Aug 16;24(16):12841. doi: 10.3390/ijms241612841

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects on eEV binding capacity after eEV storage in PBS and the EV buffer. A431 cells (EGFR-positive cells) treated with targeting (E626) EVs. (A) Representative image of eEV (gLuc) binding to A431 cells after eEV storage in PBS and the EV buffer for the indicated time. (B) The total photon flux (p/s) from EVs bound to the cells by IVIS. The value represents the means ± SD (n = 4) in the graph.