Table 4.

Summary of data obtained for the eight blinded test compounds and SC-79 using whole-cell APC, manual LPC, and SSME, as shown in Figure 8. All parameters were derived from the respective fits using the EC₅₀ equation I = I_max − (I_max − I_min)/(1 + (c/EC₅₀)ⁿ). For all fits, the adjusted R² is provided as a measure for the quality of the fit.

Cluster	Compound	Technique	EC50 (µM)	n	Imax − Imin (nA)	R²
N.A.	SC-79	APC	61.8 ± 43.2	1.17 ± 0.38	2.45 ± 1.02	0.983
		SSME 1	2.9 ± 2.3 1	0.89 ± 0.6	0.09 ± 0.04 1	0.893
		LPC	21 ± 32	1	0.91 ± 0.52	0.733
1	1	APC	10.8 ± 3.1	2.78 ± 2.1	3.96 ± 1.04	0.846
		SSME	12.9 ± 15.1	0.86 ± 0.34	0.91 ± 0.41	0.971
		LPC	3.8 ± 1.2	1	3.51 ± 0.42	0.993
	2	APC	6.5 ± 2.7	2.38 ± 1.45	0.81 ± 0.18	0.88
		SSME	30.3 ± 12.8	1.61 ± 0.81	0.74 ± 0.2	0.943
		LPC	15.9 ± 5.8	1	5.16 ± 0.7	0.981
2	3	APC	no saturation	N.A.	6.4 2	0.984
		SSME	no saturation	N.A.	0.46 2	0.969
		LPC	no effect	N.A.	N.A.	N.A.
	4	APC	9 ± 3.3	1.43 ± 0.85	2.02 ± 3.63	0.905
		SSME	70.4 ± 16.4	1.04 ± 0.08	1.21 ± 0.14	0.999
		LPC	no effect	N.A.	N.A.	N.A.
	5	APC	54 ± 28	1.21 ± 0.33	9.72 ± 3.09	0.986
		SSME	34.8 ± 4.2	2.7 ± 1.1	1.67 ± 0.17	0.993
		LPC	no effect	N.A.	N.A.	N.A.
3	6	APC	no saturation	N.A.	1.73 2	0.984
		SSME	no saturation	N.A.	0.48 2	0.984
		LPC	0.11 ± 0.07	1	1.95 ± 0.43	0.887
	7	APC	73 ± 212	0.66 ± 0.54	3.25 ± 3.67	0.963
		SSME	no saturation	N.A.	0.98 2	0.976
		LPC	0.2 ± 0.2	1	1.1 ± 0.35	0.83
	8	APC	96 ± 181	1.19 ± 0.77	4.75 ± 5.66	0.946
		SSME	9 ± 0.8	3	0.51 ± 0.03	0.985
		LPC	17.4 ± 6.6	1	2.07 ± 0.29	0.98

¹ Effect of SC-79 in SSME reflects off-target effects that are also present in the control (Figure 9F). In SSME, missing effects of SC-79 are explained due to the absence of PKB [48]. ² Since no saturation was observed, I_max could not be determined. Instead of I_max − I_min, we provide I(100 µM) − I_min.