Figure 4.
SPM suppresses cytokine secretion in UV-damaged cells in a 3D human epidermis model. (a) Schematic of UVB (250 mJ/cm2) irradiation and 8 h of treatment with indicated agents following 24 h of culture maintenance. Supernatants were harvested at 8 h and 32 h following UVB irradiation, and the cytokine concentrations were determined using ELISA. (b–d) Secretion levels of cytokines, IL-1α, TSLP, and TNFα after UVB irradiation. HC (hydrocortisone) and MF (mometasone furoate) were used as positive controls. N = 3 biological replicates. Error bars represent the mean ± SD.