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. 2023 Aug 11;24(16):12670. doi: 10.3390/ijms241612670

Figure 2.

Figure 2

RXFP1, 7BP, and binding affinities of alanine analogs of B7-33. Cartoons depicting (A) RXFP1 compared to (B) 7BP, which consists of the RXFP1 ectodomain fused to a single transmembrane domain. (C) HEK-7BP binding curves for B7-33 key residue substitutions. (D) Acetylated B7-33 analogs with alanine substitutions (in red) at key binding residues were compared across RXFP1 and 7BP, which identified 7BP as able to resolve the difference of binding due to each residue substitution. (D) Representation of B7-33 with positions of substitutions identified. (E) Peptide sequences of the analogs tested. # p < 0.001 vs. B7-33.