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Attempts to reduce the single-chain H2 relaxin scaffold sequence length. (A) Representation of H2 relaxin single-chain analog sequences. (B) pKi data for HEK-7BP binding (from C/D) and cAMP potency data; pEC50 for RXFP1 (from Figure S1) for analogs. (C) HEK-7BP binding curves for C-terminal truncations. (D) HEK-7BP binding curves for N-terminal truncations. * p < 0.05 vs. B7-33 and # p < 0.001 vs. B7-33.
