FIG. 1.

EtBr-stained agarose gel of PCR products obtained from five plasmids containing the SAP1 to SAP6 genes. DNAs from SAP1 and SAP4 were obtained from pCA1-4 as explained in Materials and Methods. Lanes: 1, molecular size marker (100-bp ladder; Promega Corporation, Madison, Wis.); 2, SAP1 DNA insert isolated and purified from pCA1-4; 3, SAP2 DNA insert from pCA2; 4, SAP3 DNA insert from pCA3; 5, SAP4 DNA insert isolated and purified from pCA1-4; 6, SAP5 DNA insert from pCA5; 7, SAP6 DNA insert from pCA6; 8, void; 9, positive control; 10, void; 11, negative control. The measured length (∼263 bp) of the PCR products corresponded to the length expected from available N-terminal sequences.