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Generation of transgenic plants. (A) SlTLP5 and SlTLP6 overexpression vector backbone construction. (B) Schematic representation of CRISPR/Cas9 cassettes used for SlTLP5 and SlTLP6 mutant vectors. The plant genome sequences of SlTLP5 and SlTLP6 were aligned via CLUSTALX nucleic acid sequence alignment, and gRNA sequences are highlighted in red.
