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. 2023 Aug 14;14(8):1622. doi: 10.3390/genes14081622

Figure 4.

Figure 4

Gene function validation via overexpression and CRISPR/Cas9 gene editing. Plants treated with bacterial and fungal pathogens were photographed on days 7 and 14 after infection for wilting disease and disease resistance identification. (A) Plants overexpressing SlTLP5 and SlTLP6 demonstrated increased disease resistance. (B) Disease grades (* indicates p < 0.05) for To-TLP5, To-TLP6, and empty plasmid transgenic plants after infection with five pathogens. (C) SlTLP5 and SlTLP6 knockouts decreased plant disease resistance. (D) Disease grading for KO-SlTLP5, KO-SlTLP6, and off-target transgenic plants after infection with the five pathogens. The error bar is the standard deviation of three biological replicates: Student’s t-test; asterisks (*) indicate p < 0.05.