Figure 7.

Effect of MP treatment on glomerular HO-1 induction in female rats. Left panel (a), capillary electrophoresis. Capillary-based separation and immunodetection of target proteins, rabbit IgG (55 kDa) and HO-1 (32 and 37 kDa), present in total protein lysates obtained from glomeruli of female rats that received anti-Fx1A Ab and assigned to metalloporphyrin (MP) treatment groups. Right panel (b), normalization of protein expression. IgG and HO-1 proteins present in each lysate were expressed as the area of RbIgG (blue bars) and HO-1 (orange bars) chemiluminescence peaks obtained in the capillary electropherogram and factored (corrected) by total protein loaded in that capillary. Ctl, control rats (n = 3, capillaries 2–4). Ab, rats receiving a single intravenous injection of rabbit anti-rat Fx1A antibody (n = 3, capillaries 5–7). AbH, rats receiving the anti-Fx1A antibody (Ab) and treated with FePPIX (hemin, H), as described in Section 4 (n = 3, capillaries 8–10). AbCo, rats receiving the anti-Fx1A antibody (Ab) and treated with cobalt protoporphyrin (CoPPIX) (n = 3, capillaries 11–13). AbPP, rats receiving the anti-Fx1A antibody (Ab) and treated with metal-free protoporphyrin (PP) (n = 3, capillaries 14–16). Capillary 1 in 7a, protein standard ladder. Numbers next to each lysate label indicate the numerical identification of lysate generated for each treatment group (Ctr, Ab, AbH, AbCo, AbPP).