Table 2.

Composition of solutions used for whole-cell patch-clamp recording.

Ion Channel	Bath Solution (in mM)	Pipette Solution (in mM)
TRPV3	139 NaCl, 5 KCl, 1.8 BaCl2, 2 MgCl2, 10 glucose, 10 sorbitol, and 10 HEPES (pH 7.4, adjusted with NaOH)	120 CsCl, 2 MgCl2, 3 Mg-ATP, 10 EGTA, 10 HEPES, and 3.15 CaCl2 (equivalent to 150 nM free Ca2+, calculated by the free Webmaxc software) (pH 7.2, adjusted with CsOH)
TRPV3 mutants		120 CsCl, 2 MgCl2, 3 Mg-ATP, 10 EGTA, and 10 HEPES (pH 7.2, adjusted with CsOH)
TRPV4	145 NaCl, 3.6 KCl, 10 HEPES, 5 glucose, 1 MgCl2, and 1.3 CaCl2 (pH 7.4, adjusted with NaOH)	140 CsCl, 1 MgCl2, 5 HEPES, 10 EGTA, and 3 Mg-ATP (pH 7.2, titrated by CsOH)
TRPA1		150 CsCl, 10 BAPTA, 10 HEPES, and 1 MgCl2 (pH 7.2, adjusted by CsOH)