Figure 1.

In binary bacterial consortia, P. copri and R. rubrum stimulated the growth of E. coli. The graphs represent the effect from the addition of 20 or 40% LB conditioned by E. coli (a–d), P. copri and R. rubrum (e–h). Bacterial growth was tracked by the change in OD₆₅₀ in different time intervals, using the areas under the dynamic curves as a sensitive integral parameter and GraphPad Prizm 5 software [60] for their calculation. Student’s t-test based on 5 (b,f) and 3 (d,h) independent biological experiments was applied for statistical assessment.