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. 1998 Feb;36(2):437–442. doi: 10.1128/jcm.36.2.437-442.1998

FIG. 2.

FIG. 2

PCR amplification of the internal region of the VanA gene cluster. (A) The vanA gene of selected isolates, from position 7266 to 7623. Isolates 12 and 18 (lanes 5 and 10) gave no positive product. (B) The vanX gene of selected isolates, from position 8129 to 8483. Isolates 12 and 18 (lanes 5 and 10) gave no positive product. (C) The vanSH intergenic region of selected isolates, from position 5715 to 6031. Isolates 12 and 18 (lanes 5 and 10) gave no positive product. Isolates 15 through 17 (lanes 7 through 9) gave a positive product of approximately 1,900 bp. The Boehringer IV (Boehringer Mannheim) was used for size determination. Sizes of markers are 2,176, 1,766, 1,230, 1,033, 653, 517, 453, 394, 298, 234, 220, and 154 bp. Results for isolates are shown as follows: lane 1, 5; lane 2, 6; lane 3, 7; lane 4, 11; lane 5, 12; lane 6, 14; lane 7, 15; lane 8, 16; lane 9, 17; lane 10, 18; lane 11, 22; lane 12, 24; lane 13, 25; lane 14, 27; lane 15, 28; lane 16, 29; lane 17, 30; lane 18, 34; lane 19, 37; lane 20, 40.