TABLE 3.
Genetic characterization of Tn1546-like elements from vancomycin-resistant enterococci by PCR and DNA hybridization
| Isolate typea | Isolate no. | Source | PCR result
|
DNA hybridization signald (kb)
|
|||||
|---|---|---|---|---|---|---|---|---|---|
| M vanXb | IS1216V–IS3-likec | IS1251c | VanR (H3) | VanX
|
|||||
| BHI-KI | BHI-BII | BHI-EV | |||||||
| 1 | 1 | Pony | G | 0 | 0 | 7 | 3.5 | 2.5 | |
| 1 | 2 | Duck | G | 0 | 0 | 7 | 3.5 | 2.5 | |
| 1 | 3 | Chicken | G | 0 | 0 | 7 | 3.5 | 2.5 | |
| 1 | 4 | Turkey | G | 0 | 0 | 7 | 3.5 | 2.5 | |
| 1 | 13 | Human | G | 0 | 0 | 7 | 3.5 | 2.5 | |
| 1 | 27 | Human | G | 0 | 0 | 7 | 3.5 | 2.5 | |
| 1 | 30 | Broiler | G | 0 | 0 | 7 | 3.5 | 2.5 | 2.1 |
| 1 | 33 | Broiler | G | 0 | 0 | 7 | 3.5 | 2.5 | |
| 1 | 34 | Broiler | G | 0 | 0 | 7 | 3.5 | 2.5 | |
| 1 | 38 | Broiler | G | 0 | 0 | 7 | 3.5 | 2.5 | |
| 2 | 8 | Pig | T | 1 | 0 | 7 | 3.5 | 2.5 | |
| 2 | 9 | Pig | T | 1 | 0 | 7 | 3.5 | 2.5 | |
| 2 | 10 | Pig | T | 1 | 0 | 7 | 3.5 | 2.5 | 2.1 |
| 2 | 14 | Human | T | 1 | 0 | 7 | 3.5 | 2.5 | |
| 2 | 28 | Human | T | 1 | 0 | 7 | 3.5 | 2.5 | 2.1 |
| 2 | 31 | Pig | T | 1 | 0 | 7 | 3.5 | 2.5 | |
| 2 | 32 | Pig | T | 1 | 0 | 7 | 3.5 | 2.5 | |
| 2 | 35 | Pig | T | 1 | 0 | 7 | 3.5 | 2.5 | |
| 2 | 36 | Pig | T | 1 | 0 | 7 | 3.5 | 2.5 | |
| 2 | 39 | Pig | T | 1 | 0 | 7 | 3.5 | 2.5 | |
| 2 | 40 | Human | T | 1 | 0 | 7 | 3.5 | 2.5 | |
| 3 | 19 | Human | G | 0 | 0 | 8 | 3.0 | 10.5 | 10.5 |
| 3 | 20 | Human | G | 0 | 0 | 8 | 3.0 | 10.5 | 10.5 |
| 3 | 21 | Human | G | 0 | 0 | 8 | 3.0 | 10.5 | 10.5 |
| 3 | 22 | Human | G | 0 | 0 | 8 | 3.0 | 10.5 | 10.5 |
| 3 | 23 | Human | G | 0 | 0 | 8 | 3.0 | 10.5 | 10.5 |
| 3 | 26 | Human | G | 0 | 0 | 8 | 3.0 | 10.5 | 10.5 |
| 4 | 5 | Human | G | 1 | 0 | 7 | 3.5 | 2.5 | |
| 5 | 6 | Human | G | 1 | 0 | 7, 9 | 3.5, 4.4 | 2.5, 3.5 | 2.1, 2.9 |
| 6 | 7 | Human | G | 1 | 0 | 7 | 3.5 | 2.5 | |
| 7 | 11 | Human | G | 0 | 0 | 8 | 3.0 | 10.5 | 2.1 |
| 8 | 15 | Human | T | 0 | 1 | 4, 5 | 3.5 | 2.5 | |
| 9 | 16 | Human | T | 1 | 1 | 4 | 3.5 | 2.5 | |
| 9 | 17 | Human | T | 1 | 1 | 4 | 3.5 | 2.5 | 2.1 |
| 10 | 24 | Human | G | 0 | 0 | 8, 11 | 3.0, 7.0 | 5.5, 10.5 | 6.8, 10.5 |
| 11 | 25 | Human | G | 0 | 0 | 15 | 4.4 | 3.5 | 2.9 |
| 12 | 29 | Broiler | G | 0 | 0 | 8 | 4.4 | 3.5 | 2.9 |
| 13 | 37 | Human | T | 0 | 0 | 7 | 3.5 | 2.5 | |
a
Isolates are divided into 13 types according to the results obtained.
b
Sequence result of PCR-amplified region of the vanX gene. T, sequence homologous to published sequence of Tn1546; G, sequence with a single-nucleotide variation (T to G) at position 8234.
c
Presence of the IS1216V–IS3-like element and of IS1251 at the published positions in the left end of Tn1546 and in the vanSH intergenic region, respectively. Symbols: 1, correct position; 0, absence of a positive PCR amplification.
d
Where two values are given, two bands were detected. Abbreviations: H3, HindIII; BHI, BamHI; KI, KpnI; BII, BglII; EV, EcoRV.