TABLE 2.

Positions and sequences of primers used for PCR amplification of the omlA gene^a

Primer	Sequence	Position (bp)
LPF	5′-AAGGTTGATATGTCCGCACC-3′	272–290
LPF1	5′-ATTGTAAACTTTAGAGCTTTATATT-3′	34–58
LPR	5′-CACCGATTACGCCTTGCCA-3′	1223–1205
LPR1	5′-ATTAAAAAGTAAAAAAGCTATCCC-3′	1312–1289
LPR2	5′-ATCTTTTACCGATGCACTATT-3′	1340–1319
LPR3	5′-TAGATGATTACGATTAATCTTATCC-3′	667–642
LPR4	5′-AAAAGTAAAAAAGCTATCCCG-3′	1308–1287

^aPositions of the primers were calculated from the published omlA gene region (9) of an A. pleuropneumoniae strain of serotype 1. The schematic positions of the primers are shown in Fig. 1.