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Agarose gel electrophoresis of PCR-amplified DNA fragments of the pbp2B gene from S. pneumoniae. Lane M, molecular size markers (in base pairs). The penicillin MICs for the isolates are as follows: 0.03 μg/ml (lane 1), 0.125 μg/ml (lane 2), 0.5 μg/ml (lane 3), 1 μg/ml (lane 4), and 2 μg/ml (lane 5).
