TABLE 2.

Sequences of oligonucleotide primers

Primera	Sequence (5′→3′)	Position in pbp2B geneb	Product length (bp) following PCR with downstream primer P6
R1	GCCTTTTCTAGGCCAATGCCGATTAC	697–722	331
R2	GCCTACGATTCATTCCCGATT	700–720	328
R3	AAATTGGCATATGGATCTTTTCCT	694–717	334
R4	GTTTTAACTAACAATTTAGAATCC	814–837	214
P5	CTGACCATTGATTTGGCTTTCCAA	346–369	682
P6	TTTGCAATAGTTGCTACATACTG	1006–1028

^aPrimers P5 and P6 are specific for pneumococci. Primers R1 to R4 are specific for penicillin-resistant pneumococci only. 

^bAccording to the published sequences of Smith and Klugman (31).