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. 2023 Aug 14;9(8):848. doi: 10.3390/jof9080848

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Confocal microscopy of the effector candidates (A) 35S::G3GFP-Ap28303 construct accumulated in the nucleus. White arrows indicate nuclei; (B) no accumulation of 35S::Ap28303-G3GFP; (C) no accumulation of 35S::G3GFP-Ap30385; (D) no accumulation of 35S::Ap30385-G3GFP; and (E) a band of the expected size for the 35S::G3GFP-Ap28303 fusion (40 KDa: 13 kDa of Ap28303 protein + 27 kDa of GFP protein). As expected, expression of the empty pGWB652 vector resulted in a band compatible with the G3GFP tag (~27 kDa).