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. 2023 Aug 14;9(8):845. doi: 10.3390/jof9080845

Figure 2.

Figure 2

The process of extracting AMF spores using the wet sieving technique (WST). (A): Mixing soil sample with distilled water using magnetic stirrer; (B): Pouring the mixture through 80 µm and 45 µm sieves; (C): Centrifugation of liquid; (D): Pouring the supernatant through the 45 µm sieve and mixing with sucrose solution; (E): The second centrifugation; (F): Transferring the su-pernatant to 45 µm sieve, washing with tap water in new collection tube.