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. 2023 Aug 14;9(8):845. doi: 10.3390/jof9080845

Figure 3.

Figure 3

The process of extracting AMF spores using the ultrasound wet-sieving technique (UWST). (A): Mixing soil sample with distilled water using magnetic stirrer; (B): Treating the mixture using ultrasound; (C): Pouring the mixture through 80 µm and 45 µm sieves; (D): Treating the mixture using ultrasound and 45 µm sieving; (E): Adding sucrose solution and centrifugation; (F): Pouring the supernatant through the 45 µm filter paper and washing with tap water; (G): Washing the filter paper with distilled water and transferring to new collection tube.