Figure 4.

The steps of AMF spore extraction and enumeration using the ultrasound centrifuge technique (UCT). (A): Separation of rhizosphere sample from solid particles (using 2 mm sieve); (B): Weighing the sample; (C): Adding the sample to the beaker and transferring the distilled water; (D): Stirring the sample in a magnetic stirrer; (E): Treating the mixture with ultrasound; (F): Transferring the mixture into falcon tubes; (G): Mixing the mixture by hand; (H): Centrifugation of the mixture; (I): The supernatant and pellet of the mixture; (J): Transferring the supernatant through the strainer into new falcon tubes; (K): Using glycerin to the circle drawn for counting on the petri dishes; (L): Transferring the spore suspension to the drawn circle and counting it under the stereo microscope.