Figure 5.

MGV1 affects the expression of BGC genes. The wild-type (WT), ∆mgv1, MGV1Oex1, and MGV1Oex6 strains were grown in NPN conditions for 24 h, total RNA was isolated, and RT-qPCR was performed with six genes that are differentially expressed in the RNA-seq analysis. TRI5, TRI6, NPS5, and NPS9 are positively regulated by MGV1 (A–D), PKS12 and GIP1 are negatively regulated (E,F) Relative expression was calculated using the Pfaffl method with EF1α (FGSG_08811) and β-tubulin (FGSG_09530) as reference genes. Bars represent a mean of three biological replicates with error bars representing standard deviation. Significance (*) was determined with Student’s t-test at p ≤ 0.05.