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. 2023 Aug 10;13(8):1717. doi: 10.3390/life13081717

Figure 1.

Figure 1

Correlation between the Ct values and SARS-CoV-2 B.1.351 RNA at eight concentrations, as analyzed using linear regression based on LoD experiments. Graphs prepared using the results of the STANDARD M kit for (A) E and (B) RdRP; the Real-Q Direct kit for (C) E and (D) RdRP; and the Allplex™ fast kit for (E) E, (F) RdRP, and (G) N. Percentage efficiency was calculated from the slope using the formula E (%) = 100 × (−1 + 10−1/slope). Circles represent the results of each test, and 24 replicates were used per concentration. The dotted line represents the LoD value. E, envelope gene; RdRP, RNA-dependent RNA polymerase gene; N, nucleocapsid gene; Ct, Cycle threshold; and LoD, limit of detection.