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. 2023 Jul 25;13(8):1622. doi: 10.3390/life13081622

Table 4.

Effects of supplementation with different sources of nitrogen on the balance of nitrogen and AA digestibility of Nellore cattle grazing low-quality forage in the dry season.

Supplement (S) 1 SEM 2 p-Value 3
Item NPN CGM Time S Time × S
 n 8 8
 N-NH3, mg/dL 24.4 23.1 0.331 <0.001 0.327 0.624
 Microbial-N, g/d 26.3 30.1 6.412 - 0.844 -
 Bacterial efficiency 4 116.9 160.7 26.89 - 0.638 -
Nitrogen balance, g/d
 Urinary-N excretion 28.2 30.6 2.636 - 0.424 -
 Fecal-N excretion 35.4 32.4 0.857 - 0.056 -
 Retained-N 14.9 −4.33 7.590 - 0.320 -
Essential amino acids (EAA) digestibility, g/kg of DM
 Arginine 592.9 598.6 20.18 - 0.626 -
 Histidine 596.9 608.0 22.82 - 0.923 -
 Isoleucine 584.0 589.8 21.73 - 0.947 -
 Leucine 599.9 639.8 24.78 - 0.822 -
 Lysine 670.3 661.1 15.63 - 0.691 -
 Methionine 700.0 695.7 21.35 - 0.322 -
 Phenylalanine 582.7 612.3 21.85 - 0.978 -
 Threonine 559.9 559.0 24.06 - 0.707 -
 Valine 569.9 580.4 21.26 - 0.871 -
Non-essential AA (NEAA) digestibility, g/kg of DM
 Alanine 522.4 559.5 30.90 - 0.792 -
 Aspartic Acid 680.8 681.4 27.80 - 0.978 -
 Cystine 652.0 683.7 21.36 - 0.952 -
 Glutamic Acid 592.7 616.6 28.92 - 0.915 -
 Glycine 555.4 542.1 24.57 - 0.825 -
 Proline 536.3 581.4 29.33 - 0.901 -
 Serine 529.1 547.3 29.97 - 0.964 -
 Tyrosine 648.6 666.7 19.41 - 0.869 -
AA total, g/kg of DM 598.8 615.7 23.25 - 0.979 -
EAA, g/kg of DM 602.9 616.0 20.53 - 0.863 -
NEAA, g/kg of DM 591.5 609.8 26.22 - 0.956 -

1 NPN = Supplementation of urea as a source of NPN (50% of the daily requirement of RDP); CGM = supplementation of corn gluten meal 60 as the source of RUP (3 g/kg of BW per day). 2 SEM = standard error of the mean. 3 Time = effects of sampling time; S = effects of supplement; and Time × S = interaction between treatment and time. 4 Calculated as g of bacterial N/kg of OM truly digested.