Fig 2. Differential gene expression for all dogs.

(A) Differentially expressed genes at time of PD as compared to baseline. Colored dots in the volcano plot represent genes that met significance criteria (n = 14) of log2fold change < or > 1.5 and padj<0.01. The heatmap was made with the same significance criteria. (B) Differentially expressed genes in dogs who progressed prior to 90 days as compared to dogs who progressed after 90 days. Colored dots in the volcano plot represent genes that met significance criteria (n = 11) of log2fold change < or > 1.5 and p<0.05. The heatmap was made using the same significance criteria. (C) When each arm was analyzed separately using p<0.05, regardless of log2fold change, there were zero overlapping significant differentially expressed genes between all three arms in dogs who progressed prior to 90 days and those who progressed after 90 days.