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. 2023 Aug 18;13(8):960. doi: 10.3390/metabo13080960

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Specnuezhenide (SPN) upregulates the protein expression of bile acid (BA) enzymes in D−galactose (D−gal) mice. (A) Immunohistochemistry (IHC) analysis of cytochrome P4507A1 (CYP7A1), cytochrome P45027A1 (CYP27A1), cytochrome P4507B1 (CYP7B1), and cytochrome P4508B1 (CYP8B1) in the liver (black arrows, n = 5). (B) The levels of CYP7A1, CYP27A1, CYP7B1, and CYP8B1 were analyzed using western blotting. The densitometric quantification of CYP7A1, CYP27A1, CYP7B1, and CYP8B1 is shown in the right panels (n = 3). Data are expressed as mean ± SD. ** p < 0.01 compared with the model (MOD) group.