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. 2023 Aug 25;9(34):eadh9570. doi: 10.1126/sciadv.adh9570

Fig. 7. Light phase activation of DMHLepR neurons induces locomotor activity.

Fig. 7.

(A and B) Representative actograms of LepR Cre animals bilaterally injected with (A) AAV-hSyn-DIO-mCherry or (B) AAV-hSyn-DIO-hM3Dq-mCherry and injected with CNO (0.3 mg/kg) at ZT6 (solid blue line) in 12:12-hour LD cycle with access to a running wheel. Pink-shaded areas represent 1 hour before injection, and blue-shaded areas represent 3 hours after injection. See fig. S11 (A and B) for actograms of all animals. (C) Light phase (day) wheel revolutions of DMHLepR mCherry and DMHLepR hM3Dq animals before, during, and after CNO injections at ZT6 in 12:12-hour LD cycle. Pink-shaded areas represent days with CNO injection. Repeated-measures two-way ANOVA; n = 5 to 6 per group; Fvirust * time(19,171) = 3.302, P < 0.001. (D) Dark phase (night) wheel revolutions of DMHLepR mCherry and DMHLepR hM3Dq animals before, during, and after CNO injections at ZT6 in 12:12-hour LD cycle. Repeated-measures two-way ANOVA; n = 5 to 6 per group; Fvirust * time(19,171) = 1.707, P = 0.0390. (E) Average wheel running activity induced by chemogenetic activation of DMHLepR neurons at ZT6 in 12:12-hour LD cycle. Color-coded time window is indicated in (A) and (B). Repeated-measures two-way ANOVA; n = 5 to 6 per group; Fvirust * time(79,711) = 2.519, P < 0.001. (F) Quantification of activity for 5 days after cessation of CNO injections. Repeated-measures two-way ANOVA; n = 5 to 6 per group; Fvirus(1,9) = 0.9519, P = 0.3547; Fvirus * time(79,711) = 1.202, P = 0.1215. Data are represented as means ± SEM. *P < 0.05; ***P < 0.001; ns, not significant.