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. 2023 Aug 25;9(34):eadh9570. doi: 10.1126/sciadv.adh9570

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Fig. 8. — (A and B) Representative actograms of LepR Cre animals bilaterally injected with (A) AAV-hSyn-DIO-mCherry or (B) AAV-hSyn-DIO-hM3Dq-mCherry and injected with CNO (0.3 mg/kg) at ~CT14 (solid blue line), under ad libitum, constant dark conditions with access to a running wheel. See fig. S12 (A and B) for actograms of all animals. (C) Twenty-four–hour total wheel revolutions of before, during, and after CNO injections at ~CT14 in constant dark condition (DD). Repeated-measures two-way ANOVA; n = 9 to 12 per group; F_{virust * time}(29,551) = 2.468, P < 0.001. (D) Average wheel running activity induced by chemogenetic activation of DMH^LepR neurons at ~CT14. Color coded time window is indicated in (A) and (B). Repeated-measures two-way ANOVA; n = 9 to 12 per group; F_{virust * time}(140,2660) = 2.114, P < 0.001. (E and F) Representative actograms of LepR Cre animals bilaterally injected with (E) AAV-hSyn-DIO-mCherry or (F) AAV-hSyn-DIO-hM3Dq-mCherry and injected with CNO (0.3 mg/kg) at ~CT22 (solid blue line). See fig. S12 (C and D) for actograms of all animals. (G) Twenty-four–hour total wheel revolutions before, during, and after CNO injections at ~CT22 in DD. Repeated-measures two-way ANOVA; n = 9 to 12 per group; F_virus (1,19) = 0.2061, P = 0.6550; F_{virus * time}(29,551) = 0.7120, P = 0.8676. (H) Average wheel running activity induced by chemogenetic activation of DMH^LepR neurons at ~CT22. Color-coded time window is indicated in (E-F). Repeated-measures two-way ANOVA; n = 8 to 12 per group; F_virus(1,18) = 8.580, P = 0.0090; F_{virus * time}(80,1440) = 2.029, P < 0.001. (I) Quantification of sustained activity for 5 days after cessation of CNO injections. Color-coded time window is indicated in (E) and (F). Repeated-measures two-way ANOVA; n = 8 to 12 per group; F_{virus * time}(80,1440) = 1.745, P < 0.001. Data are represented as means ± SEM. ***P < 0.001; ns, not significant.