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. 2023 Aug 25;9(34):eadh9570. doi: 10.1126/sciadv.adh9570

Fig. 9. DMHLepR neurons directly and functionally connect to the SCN.

Fig. 9.

(A) Schematic illustration of NMS-Cre–dependent rabies virus monosynaptic retrograde tracing. (B and C) Representative images of (B) SCN and (C) DMH from retrograde tracing strategy in (A). (D) The ZT phase of the first bioluminescence peak of SCN and liver from PER2LUC mice injected with CNO at ZT6 (control) or PER2LUC;LepR-Cre mice with hM3Dq expressed in DMHLepR neurons and injected with CNO at ZT6 (hM3Dq). Phases of sample points are shown relative to the normalized mean phase of control SCN. Two-way ANOVA with Bonferroni post hoc comparison; n = 5 to 6 per group; Ftreatment(1,19) = 5.361, P = 0.0319. (E) The ZT phase of the first bioluminescence peak of SCN and liver from control (mixture of ZT6 saline or untreated) or ZT6 leptin-injected PER2LUC mice. Phases of sample points are shown relative to the normalized mean phase of control SCN. Two-way ANOVA with Bonferroni post hoc comparison; n = 6 to 11 per group; Ftreatment(1,30) = 4.772, P = 0.0369. Control group is the same dataset as in Fig. 1B, replotted and analyzed with ZT6 leptin-treated animals. **P < 0.01.